association between endothelial selectin (e-selectin) gene polymorphisms and e-selectin level with visceral leishmaniais, in an arms-pcr based study

نویسندگان

m fallah dept. of parasitology and mycology, school of medicine, hamadan university of medical sciences, hama

a bazmani drug applied research center, tabriz university of medical sciences, tabriz, iran.

چکیده

background: in the visceral leishmaniasis (vl), parasites reside in reticuluendothelial system, mainly in macrophages. endothelial selectin (e-selectin) might play an important role in leukocyte-endothelium interactions and inflammatory cell recruitment. the aim of this study was determining e-selectin level and its polymorphism in three groups, patients, seroposi­tive and healthy individuals. methods: serum soluble e-selectin levels as well as 2 polymorphisms of e-selectin (ser128arg and leu554phe) were measured in a cohort of patients with documented vl (n=64), a healthy control group (n=74) and a seroposi­tive for vl but without any symptoms (n=81). circulation concentration of e-selectin levels was measured by elis. the amplification refractory mutation system (arms)-pcr procedure was used for detecting polymorphisms. results: the mean of e-selectin levels significantly differed between three groups ( p <0.026), and were increased in pa­tients in comparison with other groups. difference was more considerable between two groups of patients and healthy ones (patients 92.8 ng/ml; healthy individuals 71.9 ng/ml). polymorphisms were associated with soluble e-selectin levels and altogether explained 14.4%, 7.2%, and 8.7% in patients, seropositive and seronegative healthy individuals, respectively. distribution of polymorphisms of 128ser/arg and 554leu/phe among three groups was not different significantly; however, there was a considerable arrangement in distribution of ser128arg polymorphism and 128arg allele in healthy group was more than two fold of patients (55% against 20%).  conclusion: the association between soluble e-selectin levels and visceral leishmaniasis suggests that this molecule might have significant role in the inflammatory process in vl. moreover, frequency of 128arg allele in healthy group was higher than patients.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Soluble E-selectin and P-Selectin Serum Levels in Patients with Psoriasis Compared to Healthy Controls

Introduction: Psoriasis is an inflammatory skin disease in which abnormal individual immune reactivity plays an important role. Eselectin and P-selectin are adhesion molecules expressed on vascular endothelial cells in several inflammatory skin diseases, including psoriasis. The aim of the present study was to describe selected immunological changes, concerning adhesion molecules status (sEsele...

متن کامل

The Association between α4-Integrin, P-Selectin, and E-Selectin in an Allergic Model of Inflammation

In this study, we examined the relationship between the endothelial selectins (P-selectin and E-selectin) and whether they are critical for alpha4-integrin-dependent leukocyte recruitment in inflamed (late phase response), cremasteric postcapillary venules. Animals were systemically sensitized and 2 wk later challenged intrascrotally with chicken ovalbumin. Leukocyte rolling flux, adhesion, and...

متن کامل

P-selectin, and not E-selectin, negatively regulates murine megakaryocytopoiesis.

To assess the role of P-selectin and E-selectin in megakaryocytopoiesis, in vitro assays were performed in animal models deficient in both adhesion receptors. There was a significantly greater number of IL-3-responsive megakaryocyte progenitors CFU (CFU-MK) and an increase in immature megakaryoblasts in response to IL-6 in the P-selectin-null mice compared with the wild-type controls. Furthermo...

متن کامل

Antibodies cross-reactive with E- and P-selectin block both E- and P-selectin functions.

E- and P-selectin are inflammation-induced cell adhesion molecules that mediate leukocyte-endothelial cell and leukocyte-platelet interactions. Monoclonal antibodies (MoAbs) specific for either E-selectin or P-selectin are protective in several animal models of inflammatory disease. To generate an MoAb with broader therapeutic potential, MoAbs that bind to both E- and P-selectin were generated ...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
iranian journal of parasitology

جلد ۳، شماره ۴، صفحات ۲۳-۳۱

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023